[mira_talk] Re: PacBio for scaffolding?
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 19 Jul 2011 00:54:49 +0200
On Jul 18, 2011, at 23:25 , Robin Kramer wrote:
> I doubt that pacbio is going to bring much to the assembly world until that
> accuracy improves. The problem is the reads will still need to be "error
> corrected" by competing sequencing technologies(similar to the problem that
> 454 reads have),
Math is simple: if PB gets reads >= 1500 at >=95 %, 454 unpaired reads are in
danger of extinction.
I don't think that this is out of reach.
Bacteria and lower eukaryotes would then have a mix of PB CCS, 454 PE and
Solexa. Or perhaps even PB CCS, PB strobed abd Solexa ... but for that the
single read accuracy of Solexa should go >= 90%.
B.
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