Dear all, I am repeating this question, because it seems to be lost. For one dataset (454 PE) i got such messages: "The reads X, Y, Z, W have the same template and MIRA was unable to differentiate between different trials. Template information not used there." Really, there are several reads (up to 6) for one template_id, but this happens rarely (approx. 1000 of such templates). Should i just ignore this message, or should i split them to unique pairs by using the TRACE_NAME field which is different for every F/R pair? Sincerely yours Nestor -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html