Dear Bastien, We are now assembling Sanger, solexa, and 454 reads using mira_2.9.45xl, and have 3 askings. 1) We will use a mira_out.txt output for a further analysis. We are happy if MIRA makes a result txt file one by one contig, or adds a created contig to a result txt file, because we can check a part of results without waiting for finishing whole assemblings. 2) Can you estimate how long time does MIRA take for finishing an assemble 500,000 454-reads (ca. 200 Mb) with the options "-fasta -job=denovo,est,draft,454 -notraceinfo -SB:lsd=yes -SK:pr=95 -SK:mnr=yes -OUT:ort=yes"? Our machine (64bit linux, 2 GHz CPU, 128 GB memory) has been running for more than a week. 3) Please tell us a difference between conting headers, e.g., mira_c# and mira_lrc#. Regards, Kenta Shirasawa -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html