[mira_talk] Re: MIRA vs Newbler (454 est's)
- From: "Przemyslaw Gierski" <gierski@xxxxxxxxxxxxxxxxxx>
- To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
- Date: Mon, 25 Jul 2011 17:29:42 +0200
Hello,
Start with reading a MIRA manual:
http://mira-assembler.sourceforge.net/docs/DefinitiveGuideToMIRA.html
Part about 454 sequences is important:
http://mira-assembler.sourceforge.net/docs/DefinitiveGuideToMIRA.html#chap_454
Usually you need to preproces data.
After that you call mira with: mira -project=NAME_OF_PROJECT
-job=accurate,denovo,genome,454
--
With best regards, Przemyslaw Gierski.
From: mira_talk-bounce@xxxxxxxxxxxxx [mailto:mira_talk-bounce@xxxxxxxxxxxxx]
On Behalf Of Diogo Santos
Sent: Montag, 25. Juli 2011 17:19
To: mira_talk@xxxxxxxxxxxxx
Subject: [mira_talk] MIRA vs Newbler (454 est's)
Hello
I have received data from an EST's project from 454. they make the assembly
with Newbler 2.5 and they get 5869 contigs (length>100) with coverage 11x (I
know it's low, but it's what I get:( ). I try to use MIRA to make some testing
and use the original data to reassemble, but I get a strange result (19378
contigs with length >100 and coverage 4x). Can you teel wich parameters should
I change?
Thanks
Diogo Santos
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