Hello I have received data from an EST's project from 454. they make the assembly with Newbler 2.5 and they get 5869 contigs (length>100) with coverage 11x (I know it's low, but it's what I get:( ). I try to use MIRA to make some testing and use the original data to reassemble, but I get a strange result (19378 contigs with length >100 and coverage 4x). Can you teel wich parameters should I change? Thanks Diogo Santos