[mira_talk] MIRA vs Newbler (454 est's)

  • From: Diogo Santos <diogofcul@xxxxxxxxxxx>
  • To: <mira_talk@xxxxxxxxxxxxx>
  • Date: Mon, 25 Jul 2011 15:19:23 +0000

Hello
I have received data from an EST's project from 454. they make the assembly 
with Newbler 2.5 and they get 5869 contigs (length>100) with coverage 11x (I 
know it's low, but it's what I get:( ). I try to use MIRA to make some testing 
and use the original data to reassemble, but I get a strange result (19378 
contigs with length >100 and coverage 4x). Can you teel wich parameters should 
I change?

Thanks
Diogo Santos
                                          

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