On 14 May 2014, at 22:07 , Martin MOKREJŠ <mmokrejs@xxxxxxxxx> wrote: > […] > Um, for EST projects I would propose going for 2000 only. 5kb is too much. > Did you say 5kb is for genome assemblies only? ;-) As Sabrina had genome assembly specified in her manifest, I based my answer on that. > Can one decrease the 50% threshold (for EST projects …)? There’s no “large contig” data generated by MIRA for EST/RNASeq projects as this make no sense at all: contigs built for genes expressed 10000x lower than the highest expressed genes are still valid contigs (most of the time anyway). B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html