[mira_talk] Re: MIRA 3.0.5 error from Assembly::extendADS
- From: Peter <peter@xxxxxxxxxxxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Thu, 6 May 2010 10:13:46 +0100
On Fri, Apr 23, 2010 at 5:04 PM, Peter <peter@xxxxxxxxxxxxxxxxxxxxx> wrote:
> On Thu, Apr 22, 2010 at 7:56 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote:
>> Won't make a difference. Switch off read extension will do the trick here:
>> "-DP:ure=no" for Sanger (and 454 for good measure).
>
> That worked (the assembly finished), thanks :)
>
> I think I need to explore all the MIRA trim options here - I'd been
> manually trimming my 454 to remove both the MID barcode and
> our PCR primer sequence (otherwise I was finding MIRA would
> extend the reads back into the primer).
For the record, I think MIRA was actually behaving fine - I was
just confused by the Tablet assembly viewer showing the clipped
parts of the reads (as discussed on the other thread).
Peter
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