Hi Bastien, Just to let you know that I got the same error as Peter reported a few days ago. Command and last lines of the log are pasted here below. Files and reads are available at your request. Trying with -DP:ure=no now. Best, Lionel On Thu, 2010-04-22 at 18:08 +0100, Peter wrote: > in de novo mode I've had a few > of runs fail as shown below with an error from Assembly::extendADS > which I don't think has been reported before. Parsing parameters: --project=m07aNrPeM17 -fasta --job=denovo,genome,454,sanger,accurate -OUT:ora=yes -GE:not=4 -AS:urd=yes:klrs=no -SK:mmhr=5 454_SETTINGS -CO:mrpg=8:mgqrt=30:mnq=20 [...] Searching possible read extensions (for Sanger and/or 454): [0%] ....|Len1: 2789 Len2: 0 Internal logic/programming/debugging error (*sigh* this should not have happened). Please file a bug report on http://sourceforge.net/apps/trac/mira-assembler/ "len1 or len2 == 0 ?" ->Thrown: Dynamic::setSequences(const char * seq1, uint32 len1, const char * seq2, uint32 len2, bool calcwithoffset, int32 expectedoffset) ->Caught: void Assembly::extendADS(int32 version, const string prefix, const string postfix, const string logname) Aborting process, probably due to an internal error. If you want to report the error, please do so on http://sourceforge.net/apps/trac/mira-assembler/ and also give a short notice on the mira talk mailing list. If reporting, please do not delete the log and checkpoint directories, there may be files in them which could be needed to find the problem. CWD: /seq/m07a/Assembly/m07aNrPeM17 Thank you for noticing that this is *NOT* a crash, but a controlled program stop. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html