[mira_talk] Re: Is quality file necessary for assembly after qual filtering
- From: Juan Daniel Montenegro Cabrera <jdmontenegroc@xxxxxxxxx>
- To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
- Date: Wed, 22 May 2013 12:18:01 -0500
It is also important in consensus calculations and SNP mining if I remember
correctly.
El miércoles, 22 de mayo de 2013, Raj Kumar, Praveen Kumar escribió:
> Hi all,
> I kind of have a general question regarding de-novo assembly. I am
> using both Prinseq (for qual filtering) and Seqclean (adapter trimming +
> trim polyA tail + etc) for cleaning my cDNA sequences.
>
> Seqclean works only with fasta files.....so I end up with fasta sequences
> without qual (quality) scores. Is it OK to use only fasta seqs for assembly
> and anyhow I did qual filtering already? OR Is the qual scores used for
> anyother purpose in assembly other than qual filtering?. In other words
> what are the uses of qual scores in assembly other than qual filtering?
>
>
> Please let me know
>
> Thank you for reading and your help in advance,
>
> Praveen
>
>
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