[mira_talk] Is quality file necessary for assembly after qual filtering
- From: "Raj Kumar, Praveen Kumar" <rajkump@xxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Wed, 22 May 2013 11:26:52 -0400
Hi all,
I kind of have a general question regarding de-novo assembly. I am using
both Prinseq (for qual filtering) and Seqclean (adapter trimming + trim
polyA tail + etc) for cleaning my cDNA sequences.
Seqclean works only with fasta files.....so I end up with fasta sequences
without qual (quality) scores. Is it OK to use only fasta seqs for assembly
and anyhow I did qual filtering already? OR Is the qual scores used for
anyother purpose in assembly other than qual filtering?. In other words
what are the uses of qual scores in assembly other than qual filtering?
Please let me know
Thank you for reading and your help in advance,
Praveen
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