[mira_talk] Re: 500Mb assembly

  • From: "Filip Van Nieuwerburgh" <Filip.VanNieuwerburgh@xxxxxxxx>
  • To: <mira_talk@xxxxxxxxxxxxx>
  • Date: Fri, 11 Dec 2009 09:36:30 +0100

The biggest IDENTICAL repeat was 2205bp. To find this, I used the
programs UGENE and findpat. Both programs gave exactly the same results.
I must say that I am also skeptical about this result. Maybe I should
run these programs on an validation dataset. Suggestions are welcome.

Kind regards,
Filip

-----Original Message-----
From: mira_talk-bounce@xxxxxxxxxxxxx
[mailto:mira_talk-bounce@xxxxxxxxxxxxx] On Behalf Of Bastien Chevreux
Sent: donderdag 10 december 2009 19:51
To: mira_talk@xxxxxxxxxxxxx
Subject: [mira_talk] Re: 500Mb assembly

On Donnerstag 10 Dezember 2009 Filip Van Nieuwerburgh wrote:
> I checked the repeats in the most closely related genome (honey bee).
> The biggest repeat was around 2000bp.

I'm a bit sceptical here. Even bacteria have repeats larger than that
and many 
of them you don't get assembled completely with only a 3kb library. 

You've got a higher eukaryote ... how did you get to 2k as maximum
repeat 
size?

Regards,
  Bastien

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