[mira_talk] 500Mb assembly
- From: "Filip Van Nieuwerburgh" <Filip.VanNieuwerburgh@xxxxxxxx>
- To: <mira_talk@xxxxxxxxxxxxx>
- Date: Thu, 10 Dec 2009 16:39:48 +0100
Dear,
I have been asked to de novo assemble an insect genome.
Until now, I only de novo assembled bacterial genomes (max 5 Mb). Mira
proved to be the best assembler (in my hands) to tackle these
assemblies.
What would be the best strategy (also taking into account that I cannot
wait more than a month for MIRA to finish) to de novo assemble the 500Mb
insect genome?
I have:
- 100E6 reads of 2x100 bp Illumina data
- 1 full Roche titanium run: paired-end insert size= 3000 bp.
- A 16 core 64-bit linux with 128Gb of RAM
Thank you!
Kind regards,
Filip
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