On 25 Jun 2014, at 23:51 , Jose Huguet Tapia <jch63@xxxxxxxxxxx> wrote: > Running now. > I got a warning though > :WARNING!!!!!! > You are not performing a 'mapping only' assembly and the parameters > -SK:bph=6 and -SK:hss=1 > are quite low. If SKIM takes ages, stop this assembly and restart while > increasing these parameters. > > Should I continue or change the bph and hss parameters Owww, frak! I completely missed that you are running low quality PB. Stop that, it won’t work. MIRA currently can run only with high quality PB reads, i.e., corrected reads. Sorry. B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html