On Dienstag 19 Mai 2009 Schafleitner, Roland (CIP) wrote: > I tried to extract sequence, quality and traceinfo files from sff files > using sff_extract and at the same time clipping 6 bases from the left > end using the -min_left_clip=6 command in sff_extract to correct for the > wrong clipping performed by the sequence provider. MIRA however cannot > load the resulting files, as the number of bases per read in the > sequence and qual files seem not to be identical anymore. > Has anybody an idea how to overcome this problem? Hi Roland, can you please have a look at the newest version of sff_extract that Jose uploaded? (http://bioinf.comav.upv.es/sff_extract/download.html) It should solve your problem. I think you bumped into the same problem of malformed SFF files as other people did. I made a quick fix and sff_extract is now prepared for that eventuality. Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html