[mira_talk] clipping in sff_extract
- From: "Schafleitner, Roland (CIP)" <R.SCHAFLEITNER@xxxxxxxxx>
- To: <mira_talk@xxxxxxxxxxxxx>
- Date: Tue, 19 May 2009 08:11:31 -0500
Hi,
I tried to extract sequence, quality and traceinfo files from sff files
using sff_extract and at the same time clipping 6 bases from the left
end using the -min_left_clip=6 command in sff_extract to correct for the
wrong clipping performed by the sequence provider. MIRA however cannot
load the resulting files, as the number of bases per read in the
sequence and qual files seem not to be identical anymore.
Has anybody an idea how to overcome this problem?
Thank you very much,
R
Roland Schafleitner
PhD, Biotechnology Research Scientist
Germplasm Improvement and Crop Enhancement Division
International Potato Center - CIP
Avenida La Molina 1895, Apartado 1558
La Molina, Lima 12, Peru
Tel.: +51 (0) 1 349 6017, annex 3065
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