On Saturday 18 April 2009 Sucheta Tripathy wrote: > I would be interested in testing this beta version. Only thing I would like > to know is how much memory I will be needing for a genome of size approx. > 100 MB. I have a limitation with the memory usage. Hello Sucheta, this actually is something I'd like to find out myself. If I remember correctly, you had some 900k Sanger reads, right? Now, if MIRA uses more than 40 to 50 GiB, then I'd think that something happened I did not foresee. For a "quick" first test, please start MIRA like this: mira --project=... --job=genome,denovo,normal,sanger -GE:not=4 -DP:ure=no -CL:pvlc=no -SK:mchr >&log_assembly.txt (other switches at will) This starts MIRA and turns off the most time consuming tasks so that you comparatively quickly get an idea how things behave. - if you want to use more threads during skim, increase -GE:not - if you have less than 32 GiB memory, set -SK:mchr to 4092 (or less if really tight) I'm curious how it's going to run ... Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html