Hello, This is my assembly info: *Assembly information: ===================== Num. reads assembled: 194398 Num. singlets: 82 Large contigs: -------------- With Contig size >= 500 AND (Total avg. Cov >= 29 OR Cov(san) >= 0 OR Cov(454) >= 27 OR Cov(sxa) >= 0 OR Cov(sid) >= 0 ) Length assessment: ------------------ Number of contigs: 169 Total consensus: 763757 Largest contig: 48172 N50 contig size: 9852 N90 contig size: 1758 N95 contig size: 1370 Coverage assessment: -------------------- Max coverage (total): 894 Max coverage Sanger: 0 454: 900 Solexa: 0 Solid: 0 Avg. total coverage (size >= 5000): 87.13 Avg. coverage (contig size >= 5000) Sanger: 0.00 454: 88.79 Solexa: 0.00 Solid: 0.00 Quality assessment: ------------------- Average consensus quality: 78 Consensus bases with IUPAC (IUPc): 446 (you might want to check these) Strong unresolved repeat positions (SRMc): 1 (you might want to check these) Weak unresolved repeat positions (WRMc): 0 (excellent) Sequencing Type Mismatch Unsolved (STMU): 0 (excellent) Contigs having only reads wo qual: 0 (excellent) Contigs with reads wo qual values: 0 (excellent)* I would like to choose ONLY this 169 contigs to work on it in GAP4. I know how to choose contigs based on size, but how can I get this 169 contigs in ONE file? How does this results looks like... :). There 894 coverage, this is because there are repeats which pile up on each other, is there any option to "stop" MIRA of doing it? Andrzej I looked on link to your manual, but there is only way of choosing size and coverage as a selection method...