[mira_talk] MIRA Error Message
- From: Jeremiah Davie <jdavie@xxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Fri, 4 Dec 2009 14:00:10 -0500
Hi Everyone,
Quick question: In my assembly of 454 data (older GS20 data from
standard chemistry), my output log lists that it found 290768 reads
extracted from two *.sff files, yet when those reads are loaded I
receive 145339 instances of the following error message: "Error: read
name EERNOEK******* present multiple times in readpool!", where the
asterisks denote a different read name. Since this is almost half of
the the total loaded reads, I'm curious what might have caused this?
Presumably sff_extract used the names found within the *.sff files for
these reads, but it seems strange to me that the two *.sff files would
contain all the same names. Did I do some thing wrong in the
sff_extract process that might have caused this without generating an
error message? Any thoughts? Thanks in advance everyone! - Jeremiah
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