[mira_talk] More than two reads for one <template_id>

  • From: Nestor Zaburannyi <nestor@xxxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Wed, 20 Jul 2011 14:32:25 +0200

Dear all,

I am repeating this question, because it seems to be lost.

For one dataset (454 PE) i got such messages:

"The reads X, Y, Z, W have the same template and MIRA was unable to 
differentiate between different trials. Template information not used there."

Really, there are several reads (up to 6) for one template_id, but this happens 
rarely (approx. 1000 of such templates). Should i just ignore this message, or 
should i split them to unique pairs by using the TRACE_NAME field which is 
different for every F/R pair?

Sincerely yours
Nestor


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