[mira_talk] Re: FASTA WITH QUALITY VALUES
- From: Lionel Guy <guy.lionel@xxxxxxxxx>
- To: mira talk <mira_talk@xxxxxxxxxxxxx>
- Date: Tue, 25 Feb 2014 15:03:28 +0100
Yes.
From the manual
(http://mira-assembler.sourceforge.net/docs/DefinitiveGuideToMIRA.html#sect_res_resultsdir):
The *_d_results directory [...]
• projectname_out.unpadded.fasta: as above, this file contains as FASTA
sequence the consensus of the contigs that were assembled in the process, put
positions in the consensus containing gaps were removed. The computed consensus
qualities are in the corresponding projectname_out.unpadded.fasta.qual file.
Lionel
On 25 Feb 2014, at 14:58 , Chayan Roy <chayan.roy93@xxxxxxxxx> wrote:
> Hii
>
> I have performed an assembly on deep sequenced metagenome. Now i want to
> extract contigs larger than 1000. If i have to submit them in MGRAST i also
> need the quality values. I am not sure how to do this. I went through the
> manual and know how to use the convert_project to extract contigs >1000 but
> dont know how add quality values on them. Will file.unpadded.fasta.qual will
> serve my purpose??
> Any help..
>
> Mira version: MIRA V3.4.0.1 (production version).
>
> Best
> --
> CHAYAN ROY
>
>
>
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