[mira_talk] 2 common messages problems while assembly Illumina paired-end sequences
- From: "Camila M." <kamynz16@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Wed, 12 Feb 2014 09:06:13 -0500
Hello All,
As I said before, I'm doing a lot of Miras by groups of 500 Miras. I have
17 lists with 500 manifest files each.
I thougth that I didn't have any problem with the first 1500 Miras (with
the first 3 lists). However, I couldn't assembly 28 contigs of list 1, 29
contigs of list 2, and 24 contigs of list 3.
Mainly, I got these two error messages:
1) Thrown: void HashStatistics::loadHashStatisticsFile(string &
hashstatfilename, uint8 basesperhash)
2) Thrown: void Assembly::dumpSomeStatistics()
One of those manifest looks like this:
project = comp4715_c0_seq1.mira
job = est,denovo,accurate
parameters = -GE:not=1 -NW:cmrnl=no -OUTPUT:output_result_html=yes
readgroup = comp4715_c0_seq1._library_1
data =
/home/ABTLUS/maria.villa/RNASeq_Pseudozyma/read_by_contig/comp4715_c0_seq1_Glicose_1_ACTTGA_merge_R1_001.fastq
/home/ABTLUS/maria.villa/RNASeq_Pseudozyma/read_by_contig/comp4715_c0_seq1_Glicose_1_ACTTGA_merge_R2_001.fastq
technology = solexa
strain = pseudozyma
segment_placement= ---> <---
readgroup = comp4715_c0_seq1._library_2
data =
/home/ABTLUS/maria.villa/RNASeq_Pseudozyma/read_by_contig/comp4715_c0_seq1_Xilano_1_AGTCAA_merge_R1_001.fastq
/home/ABTLUS/maria.villa/RNASeq_Pseudozyma/read_by_contig/comp4715_c0_seq1_Xilano_1_AGTCAA_merge_R2_001.fastq
technology = solexa
strain = pseudozyma
segment_placement= ---> <---
readgroup = comp4715_c0_seq1._library_3
data =
/home/ABTLUS/maria.villa/RNASeq_Pseudozyma/read_by_contig/comp4715_c0_seq1_Xilose_1_GGCTAC_merge_R1_001.fastq
/home/ABTLUS/maria.villa/RNASeq_Pseudozyma/read_by_contig/comp4715_c0_seq1_Xilose_1_GGCTAC_merge_R2_001.fastq
technology = solexa
strain = pseudozyma
segment_placement= ---> <---
--
Camila M.
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