shared by Peggy This is a note from a friend who has dealt with mycoplasma...of course, I realize not everyone has access to Cornell, but there are other Vet Schools in the country who can probably give the same service. Peggy I can only tell you about what I discovered in my bitches all those years ago. Recently I have had a bitch not conceive for two consecutive breedings after having had two litters of 5 puppies with no problems. I checked her for mycoplasma which was negative. I took her back and checked her for a C&S (culture and sensitivity) and she had - well, actually, I don't recall exactly what she had but we put her on the appropriate meds and bred her again - I think she's pregnant. The moral of the story is that if you check for Mycoplasma, ALWAYS do a C&S at the same time. It does NOT take 3-4 weeks to tell if a dog has Mycoplasma. It is true that some labs are not good at growing it - apparently it is fairly delicate to grow and it has to be kept in ideal conditions. Cornell is the best place to send it. It should arrive there on Wed or Thursday in order to be set up immediately. If you have it arrive on Friday or over the weekend, it will sit and you could get a false negative result. Cornell's protocol has changed a bit over the years so you might want to contact them as to how to collect for Mycoplasma and how to ship. It used to be overnight in a cool pack - sterile swab of as far up the vaginal area as possible. I think it is still something like this but check. Jean Dodds states (and heaven knows, the woman is brilliant so I hate to say she's wrong!) that ideally the swab should be done between seasons. My recent one was done at this time, not sent to Cornell, results negative. I feel that unless this bitch had both Mycoplasma as well as the bug they found, the lab was probably right that she was Mycoplasma neg since she is now pregnant having been placed on the appropriate meds. (I WILL find out what bug it was - it is annoying me that I cannot recall - as the meds was an unusual one to have her on so you should know this). Anyway, the reason I say that I hesitatingly say that I do not agree with Jean Dodds as stated above, is that the very first bitch of mine who had Mycoplasma heavy growth which disallowed her to get pregnant so many years ago, was placed on first erythromycin - no change. Then on Chloro - negative for Mycoplasma. She came in season and we checked her when she was IN SEASON - heavy, heavy growth mycoplasma. The vet told me that she thought the Chloro had taken care of the vaginal area but did not penetrate deep into the uterus so when she came in season, all the mycoplasma started spilling out of the uterus. We then put her on Baytril and that cleared it up enough for her to get pregnant. I did not re-check her at all after that - I simply placed her on 3-4 weeks of Baytril immediately before she was due in season. When she came in season, I took her off it and bred her. She had 5-6 puppies each time after not having any puppies at all for 2 years. Having said that, I had a situation reasonably recently where a puppy was born kinda mummified. I took the bitch in to have her mycoplasma checked but the vet told me that it will likely be sent back as they cannot locate the mycoplasma with all the discharge from the birth - and that's exactly what happened. Again, I did not send it to Cornell. Personally, I just do not understand why this would be the case as they have to GROW mycoplasma. Bottom line - best to send it to Cornell if you really do suspect mycoplasma. I did not really suspect it but wanted to check anyway. Never did get an answer and the bitch has now had another litter so she should be okay and if it was mycoplasma, she would not have become pregnant. I DO think Mycoplasma comes back although I cannot guarantee that because my original bitch with the heavy growth was bred successfully, I did not bother checking her again - just kept giving her the Baytril prior to breeding her. She only had one other litter anyway so there was no point except for my own info. Nowadays, Mycoplasma tests are about $100 or more. It can get costly. I suppose if it does come back, it probably cannot be "cured" for life with antibiotics but Baytril can keep it at a dull roar, enough to get the bitch pregnant and a viable litter of puppies. Keep in mind that with low growth or even medium growth Mycoplasma the bitch can still conceive and produce a normal litter. I do not believe the animal needs to be removed from a breeding program. Yes, perhaps when the animal with mycoplasma heavy growth sits down outside and another bitch in season sits down on top of it or the infected bitch is bred and then that male is bred to another bitch, it could probably spread like wildfire in a multi dog environment however males usually (unless they happen to have a slightly compromised immune system) do not have a sterility problem with Mycoplasma - they may however harbor it and there is no question that a male could pass it along to a bitch. In fact, a repro specialist stated that a male can transfer bacteria to a bitch which in turn could send them into a pyo - probably a bitch with a compromised immune system too - but it CAN happen. I liken the problem with Mycoplasma with intestinal parasites in multi dog families. With all the number of dogs I have plus the boarding dogs that come and go, I have had some worm issues. I have a microscope and slides and flotation things and yes, I could check stools but, quite frankly, it is just easier to keep deworming them. I have found that if I keep deworming them with Panacur 3 times a year, eventually the cycle is broken and it keeps the worming problem down. Then I stop worming quite so often and still no worms but eventually I'll get them back again via the numbers of dogs. We pick up daily obviously but it's difficult with multiple dogs as you must know. The same is true with Mycoplasma. I got to the stage that I was simply estimating when the bitches were due in season, counting back 4 weeks in the event they came in early, and starting them on Baytril. I took a 60mcg tablet and quartered them - good for a 15 lb dog. Maybe a little low dosage for the 17 - 20 lbs bitches but I don't have many of those - nonetheless, it seemed to work. I got large litters and they did not miss. Now I don't do that - I have had two bitches miss so I may have to go back to it. It just gets so expensive to check for Mycoplasma - not only that, but I worry about false negative and false positive results so I would insist on it going to Cornell if I was really worried about it. Easier just to start them on Baytril. So that's what I do from time to time. This past bitch, mind you ,STILL did not become preganant - hence I checked C&S. I must say though, some years ago my vet called to ask this following question. Had I seen any bitches have viable litters then at 4 yrs never have any further litters? I had not at that time. Interestingly, she was referring to a breeder client with GSDs. Mother dog had two viable litters, now at over 4 yrs, cannot get her preganant again. Daughter did exactly the same thing. Now this is the same vet who helped me with my original bitch with mycoplasma so I felt she was pretty much on the ball with repro issues - plus she was a breeder herself at that time. So, the bottom line, after all this long explanation, is to be sure the male is fertile, the bitch is being bred at the RIGHT time and if all that is ideal and still no puppies, then do a C&S, start her on an antibiotic if there is a heavy growth bug in the C&S results, if not, then start the Baytril for a good 3 -4 weeks, take her off as soon as she comes in season, breed her and see what happens. I know of another vet who always puts his bitches on Amoxi after the Baytril for about 3 weeks into her preganancy. I have not necessarily done that but I suppose it wouldn't hurt. ============================================================================ POST is Copyrighted 2005. All material remains the property of the original author and of GSD Communication, Inc. 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