Well, I am no expert, but why the big surprise? Haven't we known for quite
sometime that virus have "shaped' the evolution of bacteria and even our own
mitochondria? Why is it a big deal that they can interact with our DNA when
infected, but specially with these franken shots, A.K.A., mRNA vaccines which
are designed to do just that by creating little RNA factories inside our cells
to produce spike proteins?
https://www.sciencefocus.com/the-human-body/virus-human-evolution/
A true lover of wisdom has hands too busy to hold on to anything! He learns by
doing and every pebble in the path becomes her teacher! Oink
On Friday, May 7, 2021, 08:03:58 AM EDT, Mouse.com> wrote:
https://www.sciencemag.org/news/2021/05/further-evidence-offered-claim-genes-pandemic-coronavirus-can-integrate-human-dna
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Lab studies of genetically engineered human cells suggest the RNA (blue) of
SARS-CoV-2 could convert to DNA in infected people and slip into their
chromosomes.
CLAUS LUNAU/SCIENCE SOURCE
Further evidence supports controversial claim that SARS-CoV-2 genes can
integrate with human DNA
By Jon CohenMay. 6, 2021 , 2:45 PM
Science’s COVID-19 reporting is supported by the Heising-Simons Foundation.
A team of prominent scientists has doubled down on its controversial hypothesis
that genetic bits of the pandemic coronavirus can integrate into our
chromosomes and stick around long after the infection is over. If they are
right—skeptics have argued that their results are likely lab artifacts—the
insertions could explain the rare finding that people can recover from COVID-19
but then test positive for SARS-CoV-2 again months later.
Stem cell biologist Rudolf Jaenisch and gene regulation specialist Richard
Young of the Massachusetts Institute of Technology, who led the work, triggered
a Twitter storm in December 2020, when their team first presented the idea in a
preprint on bioRxiv. The researchers emphasized that viral integration did not
mean people who recovered from COVID-19 remain infectious. But critics charged
them with stoking unfounded fears that COVID-19 vaccines based on messenger RNA
(mRNA) might somehow alter human DNA.
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The critics also presented a brace of scientific criticisms, some of which the
team addresses in a paper released online today by the Proceedings of the
National Academy of Sciences (PNAS). “We now have unambiguous evidence that
coronavirus sequences can integrate into the genome,” Jaenisch says.
SARS-CoV-2, the virus that causes COVID-19, has genes composed of RNA, and
Jaenisch, Young, and co-authors contend that on rare occasions an enzyme in
human cells may copy the viral sequences into DNA and slip them into our
chromosomes. The enzyme, reverse transcriptase, is encoded by LINE-1 elements,
sequences that litter 17% of the human genome and represent artifacts of
ancient infections by retroviruses. In their original preprint, the researchers
presented test tube evidence that when human cells spiked with extra LINE-1
elements were infected with the coronavirus, DNA versions of SARS-CoV-2’s
sequences nestled into the cells’ chromosomes.
Many researchers who specialize in LINE-1 elements and other “retrotransposons”
thought the data were too thin to support the claim. “If I would have had this
data, I would have not submitted to any publication at that point,” says
Cornell University’s Cedric Feschotte, who studies endogenous retrovirus chunks
in the human genome. He and others also said they expected higher quality work
coming from scientists of the caliber of Jaenisch and Young. In two subsequent
studies, both posted on bioRxiv, critics presented evidence that the supposed
chimeras of human and viral DNA traces are routinely created by the very
technique the group used to scan for them in chromosomes. As one report
concluded, the human-virus sequences “are more likely to be a methodological
product, [sic] than the result of genuine reverse transcription, integration
and expression.”
In their new paper, Jaenisch, Young, and colleagues acknowledge that the
technique they used accidentally creates human-viral chimeras. “I think it’s a
valid point,” Jaenisch says. He adds that when they first submitted the paper
to a journal, they knew it needed stronger data, which they hoped to add during
the review process. But the journal, like many, requires authors to immediately
post all COVID-19 results to a preprint server. “I probably should have said
screw you, I won’t put it on bioRxiv. It was a misjudgment,” Jaenisch says.
In the new PNAS paper, the team provides evidence that artifacts alone can’t
explain the detected levels of virus-human chimeric DNA. The scientists also
show that portions of LINE-1 elements flank the integrated viral genetic
sequence, further supporting their hypothesis. And they have collaborated with
one of the original skeptics, Stephen Hughes of the National Cancer Institute,
who suggested an experiment to clarify whether the integration was real or
noise, based on the orientation of the integrated viral sequences relative to
the human ones. The results support the original hypothesis, says Hughes, a
co-author of the new paper. “That analysis has turned out to be important,” he
says.
“The integration data in cell culture is much more convincing than what was
presented in the preprint, but it’s still not totally clean,” says Feschotte,
who now calls Jaenisch’s and Young’s hypothesis “plausible.” (SARS-CoV-2, he
notes, can also persist in a person for months without integrating its genes.)
The real question is whether the cell culture data have any relevance to human
health or diagnostics. “In the absence of evidence of integration in patients,
the most I can take away from these data is that it is possible to detect
SARS-CoV-2 RNA retroposition events in infected cell lines where L1 is
overexpressed,” Feschotte says. “The clinical or biological significance of
these observations, if any, is a matter of pure speculation at this point.”
Jaenisch’s and Young’s team do report hints of SARS-CoV-2 integration in tissue
from living and autopsied COVID-19 patients. Specifically, the researchers
found high levels of a type of RNA that is only produced by integrated viral
DNA as the cell reads its sequence to make proteins. But, Young acknowledges,
“We do not have direct evidence for that yet.”
Harmit Malik, a specialist in ancient viruses in the human genome at the Fred
Hutchinson Cancer Research Center, says it’s a “legitimate question” to ask why
people who should have cleared the virus sometimes have positive polymerase
chain Reaction tests for its sequences. But he also remains unconvinced that
the explanation is integrated virus. “Under normal circumstances, there is so
little reverse transcription machinery available” in human cells, Malik says.
The controversy has grown decidedly more civil since December. Both Young and
Jaenisch say they received more intense criticism for their preprint than any
studies in their careers, in part because some researchers worried it played
into the hands of vaccine skeptics spreading false claims about the newly
authorized mRNA vaccines. “If there ever was a preprint that should be deleted,
it is this one! It was irresponsible to even put it up as a preprint,
considering the complete lack of relevant evidence. This is now being used by
some to spread doubts about the new vaccines,” Marie-Louise Hammarskjöld, a
microbiologist at the University of Virginia, posted in a comment on bioRxiv at
the time.
And what of the original journal submission? “They rejected it,” Jaenisch says.
Posted in:
- Health
- Coronavirus
doi:10.1126/science.abj3287
