On Thu, May 22, 2014 at 3:24 PM, Bastien Chevreux <bach@xxxxxxxxxxxx
<mailto:bach@xxxxxxxxxxxx>> wrote:
On 22 May 2014, at 22:12 , Laurent MANCHON
<lmanchon@xxxxxxxxxxxxxx <mailto:lmanchon@xxxxxxxxxxxxxx>> wrote:
> […]
> arghhh, not so easy,
> i have one reference sequence (HIV genome sequence)
> second i have 2 cDNA libraries 454: libA_treated and
libB_not_treated
> we know that we have some RNA spliced in libA
> but we don't know how many and which.
> The problem is to analyze this differential expression between
libA and libB and
> and highlight the spliced RNAs.
> How to procced ? --> map libA on HIV genome, then map libB
> and finally compare the two resulting sam files if you can find
information on splicing ?
Indeed … not see easy and I am out of options here. I’d probably
need to see the data and play around with it to get a feeling of
what the best approach could be, but … nope, I’m not gonna do that :-)
B.
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Chris Hoefler, PhD
Postdoctoral Research Associate
Straight Lab
Texas A&M University
2128 TAMU
College Station, TX 77843-2128
