[mira_talk] Re: solexa paired-end several libraries
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 24 Nov 2009 18:15:14 +0100
On Dienstag 24 November 2009 Brian Forde wrote:
> what does
> " No negative values found in FASTA quality file of solexa data??"
> mean
That you should use -LR:ssiqf=no
Old Solexa quality data also had negative and had a slightly different scaling
than 'phred' values, therefore MIRA had to scale that back into something
'normal'.
The new pipelines deployed by Illumina now don't do this anymore, I think I'll
set this parameter to 'no' as default for the 3 release (or even drop the code
completely).
Regards,
Bastien
--
You have received this mail because you are subscribed to the mira_talk mailing
list. For information on how to subscribe or unsubscribe, please visit
http://www.chevreux.org/mira_mailinglists.html
Other related posts:
