[mira_talk] Re: [mira_talk] problem in assembly

  • From: "Abhishek sharma" <abhishek_btbin@xxxxxxxxxxxxxx>
  • To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
  • Date: 9 Apr 2012 08:23:21 -0000


actaully during extraction time sff_extract doesnt extract files into fastq 
properly so thatzz why it show message of input parametrizastion.

thanks .


From: Bastien Chevreux &lt;bach@xxxxxxxxxxxx&gt;
Sent: Fri, 06 Apr 2012 00:34:43 
To: mira_talk@xxxxxxxxxxxxx
Subject: [mira_talk]  problem in assembly
On Apr 5, 2012, at 9:10 ,&nbsp;Abhishek sharma wrote:

&gt; No quality data found: (454) GYFWTOJ02JFJCK

&gt; [...]

&gt; 

&gt; Fatal error (may be due to problems of the input data or parameters):

&gt; 

&gt; "Some reads had no quality values given (see log above),

&gt; please check your input data.

&gt; If sure that this is ok for your data, switch off this check with 
-AS:epoq=no

&gt; for any sequencing type you wish (Sanger, 454, IonTorrent, PacBio, Solexa, 
...)"

&gt; 

&gt; please give me solution fr that &nbsp;i have files.xml .qual .fastq .fasta 





So ... did you check whether the quality file is loaded? And if yes, did you 
check the quality file for the sequences MIRA admonishes?



B.

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