Bastien, I recently did a de novo metagenomic assembly on 684 million Illumina reads through a number of steps. From the process I got 2,634,203 contigs with a N50 of >500 and largest contig 93,991. I am now attempting a hybrid assembly with some 454 libraries. As you can see contig size is going to be a bit of an issue. I have been doing a bit of looking at the fasta2frag script and am trying to figure out what my best options are to select. Would you have any suggestions. Thanks Dallas