They are ambiguous consensus base calls, IUPAC-coded (see table 1 in http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html)For example, S stands for "strong" (G or C), Y for pYrimidine, M for aMino (A or C), K for Keto (G or T), etc...
On 8 Jul 2010, at 14:57 , Laurent MANCHON wrote:
--Hello why i obtain assemblies with letters other than ATGC: CSTASAYCGGAAGAGCTCGTATGCGGTCTTCTGTTKGAAMMMAM*AG what does it mean S,M,K, Y??? Laurent --
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