Hi Bastien, I have two queries: Can I specify the file format in the manifest (since Galaxy stores all user data in files with the extension .dat, it would be useful if I could tell MIRA that these particular files are FASTQ). Second, shouldn't there be a non-zero return code in the following failed example? I seem to be getting zero indicating success :( Thanks, Peter -- $ more example.manifest project = MIRA job = denovo,genome,accurate parameters = -GE:not=1 -DI:trt=/tmp readgroup technology = solexa data = /mnt/galaxy/galaxy-central/database/files/000/dataset_193.dat $ ~/downloads/mira_4.0rc2_linux-gnu_x86_64_static/bin/mira example.manifest This is MIRA 4.0rc2 . Please cite: Chevreux, B., Wetter, T. and Suhai, S. (1999), Genome Sequence Assembly Using Trace Signals and Additional Sequence Information. Computer Science and Biology: Proceedings of the German Conference on Bioinformatics (GCB) 99, pp. 45-56. To (un-)subscribe the MIRA mailing lists, see: http://www.chevreux.org/mira_mailinglists.html After subscribing, mail general questions to the MIRA talk mailing list: mira_talk@xxxxxxxxxxxxx To report bugs or ask for features, please use the SourceForge ticketing system at: http://sourceforge.net/p/mira-assembler/tickets/ This ensures that requests do not get lost. Compiled by: bach Sun Sep 22 16:20:04 CEST 2013 On: Linux vk10464 2.6.32-41-generic #94-Ubuntu SMP Fri Jul 6 18:00:34 UTC 2012 x86_64 GNU/Linux Compiled in boundtracking mode. Compiled in bugtracking mode. Compiled with ENABLE64 activated. Runtime settings (sorry, for debug): Size of size_t : 8 Size of uint32 : 4 Size of uint32_t: 4 Size of uint64 : 8 Size of uint64_t: 8 Current system: Linux ppserver 2.6.32-358.11.1.el6.x86_64 #1 SMP Wed Jun 12 03:34:52 UTC 2013 x86_64 x86_64 x86_64 GNU/Linux Looking for files named in data ...Pushing back filename: "/mnt/galaxy/galaxy-central/database/files/000/dataset_193.dat" Could not determine filetype from data '/mnt/galaxy/galaxy-central/database/files/000/dataset_193.dat' ("/mnt/galaxy/galaxy-central/database/files/000/dataset_193.dat") Fatal error (may be due to problems of the input data or parameters): ******************************************************************************* * Some 'data' entries named in the manifest file could not be verified, see * * the log above. * * Maybe some files are missing, not readable or there is a typo in the * * manifest file? * ******************************************************************************* ->Thrown: void Manifest::loadManifestFile(string & mfilename) ->Caught: main Aborting process, probably due to error in the input data or parametrisation. Please check the output log for more information. For help, please write a mail to the mira talk mailing list. Subscribing / unsubscribing to mira talk, see: //www.freelists.org/list/mira_talk CWD: /mnt/galaxy/pico_galaxy/tools/mira_4_0 Thank you for noticing that this is *NOT* a crash, but a controlled program stop. Failure, process aborted. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html