Hi Bastein, The new Illumina software outputs the quality value in offset of 33 ASCII as against 84 ASCII offset. Do you think it will affect my cleaning quality if I give following CL:qc=yes:qcmq=5:qcwl=5 and what might be the solution for this? Thanks, A -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html