[mira_talk] Re: PROJ_default.(un)padded.fasta vs PROJ_ReferenceStrain vs PROJ_AllStrains

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Mon, 17 Oct 2011 21:24:05 +0200

On Oct 17, 2011, at 21:13 , Bastien Chevreux wrote:
> So, assume you mapped a strain against a backbone, and you get in the 
> multiple alignment:
> 
> ReferenceStrain         gtacg*tcagtcg
> default/StrainX  read1  gtacgAtTag*cg
> default/StrainX  read2  gtacgAtTag*cg
> default/StrainX  read3  gtacgAtTag*cg
> etc.pp
> 
> (note the differences)
> 
> Now, if you extract a consensus, convert_project will create one consensus 
> sequence for each strain and one for all strains together. In the case above 
> it will be three sequences:
> 
> ReferenceStrains:   gtacgtcagtcg
> default/StrainX:    gtacgAtTagcg
> AllStrains:         gtacgWYWRKcg

Gah, wrong "AllStrains". Here's the correct one:

AllStrains:      gtacgatyagtcg

B.

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