On Donnerstag 25 Juni 2009 Björn Nystedt wrote: > [...] > I think the megahub is actually an artefact; the single read in the megahub > logfile is a long (15kb) fake read comprising a complete PCR product. (The > complte run is 300000 GS20, 16000 Sanger and ~20 PCR fake reads) Ah ... the fake PCRs is some crucial info which was missing. Yes, that explains a bit the behaviour. You sure you don't want to do a mapping assembly? Or a combined mapping and de-novo assembly? Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html