On Mon, Apr 6, 2015 at 5:08 PM, Hernan Vazquez Miranda <miran050@xxxxxxx> wrote:
Hello everybody,
I've been fighting with the following problem trying to extract the
mitogenome of the recently deposited woodpecker genome in Genbank. I
downloaded one full lane, merge all pair-end reads, and concatenated them in
a single file. I had to subsample the full file to 10% because it wouldn't
run (Killed:9 error). Now it doesn't die but Mira won't work. ...
Loading data from FASTQ file:
/Users/Odontodactylus/Sanger_data/melanerpes/downyS10.fastq
(sorry, no progress indicator for that, possible only with zlib >=1.34)
Read SRR949787.4.1: invalid quality 170
Read SRR949787.20.1: invalid quality 171
Read SRR949787.20.1: invalid quality 167
... 4GB od these error lines
Read SRR949787.119142778.1: invalid quality 171
Read SRR949787.119142778.1: invalid quality 167
Read SRR949787.119142778.1: invalid quality 165