[mira_talk] Re: Help to a new user of MIRA

  • From: Nestor Zaburannyi <nestor@xxxxxxxxxxxxx>
  • To: Bastien Chevreux <mira_talk@xxxxxxxxxxxxx>
  • Date: Fri, 1 Jul 2011 15:41:54 +0200

Dear Bastien,

> I usually need only a couple of minutes to do that by hand on the genomes I 
> work on
Can you explain please in two words the main principle?

> A very quick way: the template display in gap5 should appease your fears.
Appeased, indeed. Thanks.

I will try to deal with Bambus as Juan recommended and i'll report results. In 
the meantime, i encountered another problem. After adding additional dataset, 
this kind of error emerges:

"The reads X, Y, Z, W have the same template and MIRA was unable to 
differentiate between different trials. Template information not used there."

There are really several reads (up to 6) for one template_id, but this happens 
rarely (approx. 1000 of such templates). Should i just ignore this message, or 
should i split them to unique pairs by using the TRACE_NAME field which is 
different for every F/R pair?

Sincerely yours
Nestor


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