Dear Bastien, > I usually need only a couple of minutes to do that by hand on the genomes I > work on Can you explain please in two words the main principle? > A very quick way: the template display in gap5 should appease your fears. Appeased, indeed. Thanks. I will try to deal with Bambus as Juan recommended and i'll report results. In the meantime, i encountered another problem. After adding additional dataset, this kind of error emerges: "The reads X, Y, Z, W have the same template and MIRA was unable to differentiate between different trials. Template information not used there." There are really several reads (up to 6) for one template_id, but this happens rarely (approx. 1000 of such templates). Should i just ignore this message, or should i split them to unique pairs by using the TRACE_NAME field which is different for every F/R pair? Sincerely yours Nestor -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html