If you have short reads (1 line seq) you can write: grep -A1 '^@' in.fastq | grep -v '^-' | sed 's/^@/>/' > out.fasta And grep -A1 '^+' in.fastq | grep -v '^-' | sed 's/^+/>/' > out.fastq From: mira_talk-bounce@xxxxxxxxxxxxx [mailto:mira_talk-bounce@xxxxxxxxxxxxx] On Behalf Of Torsten Thomas Sent: Thursday, September 24, 2009 4:22 AM To: mira_talk@xxxxxxxxxxxxx Subject: [mira_talk] Fastaq to fasta and qual Hi. Does anybody have a script that converts a fastaq file to a corresponding fasta and fasta.qual file? And if yes, are you willing to share it. :-) Cheers Torsten Dr. Torsten Thomas Senior Research Fellow Centre for Marine Bio-Innovation School of Biotechnology and Biomolecular Sciences The University of New South Wales Sydney, NSW 2052 Australia Ph + 61 (0) 2 938 53467 Mob +61 (0) 410159525 Fax + 61 (0) 2 938 51779 "Anyone who believes exponential growth can go on forever in a finite world is either a madman or an economist" (Kenneth Boulding, economist) This mail was received via Mail-SeCure System. This mail was sent via Mail-SeCure System.