[mira_talk] Re: Collecting DNA from bacterial cultures, was: suggestion for sequencing companies

  • From: Peter Cock <p.j.a.cock@xxxxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Fri, 13 Apr 2012 10:10:01 +0100

On Fri, Apr 13, 2012 at 9:57 AM, Mcnally, Alan <alan.mcnally@xxxxxxxxx> wrote:
> Apologies all
>
> In this paper Paul's group used late log cells for their extractions
> and spotted a shift in the position of the terminus of replication.
> Paul has told me in meetings about the smile effect in coverage
>
> http://www.ncbi.nlm.nih.gov/pubmed/21962024
>

Ah - I skimmed the Okinaka et al 2011 paper (and a couple more
from the group), but sadly the coverage variation effect wasn't
mentioned, which was a shame - but presumably not relevant to
the main message. Thanks for the clarification.

How long before sequencing is cheap enough to use directly to
explore the dynamics of bacterial DNA replication? It seems a
bit of a sledge hammer, but you could have some fun with multiple
plasmids or looking at mutation effects.

Peter

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