[mira_talk] Beginner Question RE sanger data in hybrid assembly
- From: Jeremiah Davie <jdavie@xxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Mon, 23 Nov 2009 13:26:36 -0500
Hi All,
I am learning to use MIRA and wanted to incorporate Sanger data
along with a 454 run. The problem is that the sanger data is saved as
*.ab1 files, as the sanger sequencing facility at our university uses
an older ABI sequencer. I can use Sequencher to convert those files to
fasta/fastq files, but cannot generate the traceinfo.xml files that
MIRA expects. Is there a way to avoid using the traceinfo.xml files?
Conversely, does anyone know of a program that will convert an .ab1
file to fasta/fastq/traceinfo.xml collection? If not, can someone
guide me to an easy to follow guide for writing a traceinfo.xml file?
Any help would be greatly appreciated; I'm pulling my hair out on
this. Sincerely, Jeremiah
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