[mira_talk] Re: Assembling 454 with mira3
- From: Peter <peter@xxxxxxxxxxxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Fri, 2 Jul 2010 10:25:05 +0100
On Fri, Jul 2, 2010 at 10:11 AM, Philip Stevens <ph.stevens@xxxxxx> wrote:
> Hi all,
>
> i am little dissapointed cause my sequencer told me that the propability to
> get the fastq or sff files of my sequencing is very low. So i will need some
> help to get best results with fasta only sequencing.
That is unfortunate. The sequencing centres we've used in the UK have all
offered the SFF files (and FASTA files and QUAL files). In future I would make
sure you ask the sequencer before hand to provide the SFF file.
> here is my position:
>
> i have about 32k 454 Reads to assemble but dont have any fastq or sff
> files. So what are best parameters?
I don't know - I've not had to try this. Bastien would be the man to say.
Peter
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