Dear all, in the last announcement regarding the development versions I wrote this: These snapshots will not contain the latest bleeding edge new developments I am currently working on / testing out, so they should be relatively stable as they most of the time went through a couple of projects on my home machine already. Well, I changed my mind. In future (starting right now), some snapshots *may* contain code which needs more testing by a wider audience. However, these versions should be stable enough as I am also using them myself on a day to day basis. Version 3.2.1.7 is such a release: I merged two development branches into the main branch as part of some wider restructuring of MIRA to get it to assemble projects in the 50m to 100m reads range. For this version I'm interested in feedback on three things: - what decrease of memory usage do you see between older version and 3.2.1.7. E.g., in a project with 800k 454 FLX reads (45x coverage), it goes down from 7.1 GiB to 5.7 GiB. - are there significant changes in assembly metrics like number of large contigs etc.? That is, are there joins MIRA suddenly missed? - does MIRA stop or segfault where it did not in the past? The main changes for 3.2.1.7: - new routines to choose and trim down SKIM hits. Save tremendous amount of disk and memory for high coverage projects (coverages >50x), especially when coverages reach >100x. - new SKIM chooser also should lead to less misassemblies for those rare cases they still occure. - fixed bug which made MIRA misestimating overlap scores ind some cases. - fixed bug which made MIRA misinterpret partial overlaps as complete overlaps. - fixed memery allocation bug which led MIRA to perform a couple of more allocations than necessary. - fixed possible division by 0 (entered in 3.2.1.6) - temporary fix for error message "Asked for elements in start cache while not using startcache?" (introduced 3.2.1.5). - convert_project: -n now also works with read collections, i.e., not only on contig names when loading contigs, but reads when loading data without contigs (like in all FASTA, FASTQ, but also CAF, MAF when no contigs are defined)