On Mittwoch 03 März 2010 Laurent MANCHON wrote: > i use the same version of mira to assemble 454 reads and it's work fine > for me with good results. > this is the command line i use: > > bin/mira -project=trimmed_NG-5114_Hemo_sequence > -fasta=trimmed_NG-5114_Hemo_sequence.fna -job=denovo,est,normal,454 > -notraceinfo COMMON_SETTINGS -GENERAL:kcim=yes,not=4 -CL:pec=no > -SK:mnr=yes,nrr=8,mmhr=6 -CO:asir=yes,mr=no 454_SETTINGS -LR:wqf=no > -CO:fnicpst=yes,rodirs=10 > -CL:cpat=no,pvlc=no,qc=no,bsqc=no,mbc=no,emlc=yes,mlcr=0,smlc=0,emrc=yes,mr > cr=0,smrc=0 -OUT:sssip=no > -AL:bip=5,bmin=10,mrs=98:egp=yes:egpl=reject_codongaps:megpp=100,mo=60 > -AS:mrl=30,bdq=30,epoq=no -SK:mnr=yes:nrr=8 does the magic for you :-) Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html