On Friday 24 April 2009 Andreas Petzold wrote: > I may have found another bug in version 2.9.43 ? I want to assemble 1700 > Sanger ESTs and mira tells after clipping me that all sequences have only 0 > good bases and then crashes with a floating point exception (division by > zero ?). Looks like it. I think I know where it happened and have made a fix. > The ESTs are in a fasta file (no qualities available). I start mira with: > mira -project=clap -fasta=clap_in.fasta -job=denovo,est,normal,sanger > -notraceinfo > > If I deactivate clipping with '-noclipping' then all works fine. Also if I > use 2.9.39 ... > > Have I missed something ? Not really, but I need to really handle missing qualities differently. Too many mails regarding this subject show me that most users are unaware that parts of their data could be completely clipped away. I think I'll make a strict rule which stops assembly if reads are missing qualities that can be overridden by a parameter ... but with the stern warning that users are then taking risks. Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html