Maybe it is not something common to post a message of this kind, but i really appreciate your attention and rapid response Mr.Chevreux. In fact i am a member of seqanswers and reading a lot from it. Thank you, i will be following this talk-list, maybe some day i might answer a question, Visam GULTEKIN > From: bach@xxxxxxxxxxxx > To: mira_talk@xxxxxxxxxxxxx > Subject: [mira_talk] Re: mira- assembly jobs > Date: Sat, 5 Mar 2011 17:11:02 +0100 > > On Wednesday 02 March 2011 12:42:58 Visam Gültekin wrote: > > [...] > > I am wondering what are the basic steps of this area, what kind of > > instructions to follow, etc..? There are so many questions and most of > > them should be answered by my own effort of course, but where to dig to > > find the treasure? > > Actually an intersting question, I had hoped a couple of other people to come > forward to say how they learned stuff about this topic. As no one else does, > I'll start. But please be advised that part of the following is just a > personal opinion. > > > I am trying to read articles about the topic i am asking, but there are so > > many gaps. One tells about north, another from south. There might be some > > cookbooks, some recipes that can be followed i think. > > Well, you basically hit the nail on the head with that observation: like > cookbooks, recipes for sequence assembly come in tons of different flavours > and > there are a multitude of them. Unfortunately, unlike cookbooks, many of the > underlying technologies change so rapidly that recipes which were valid 15 > years ago (or sometimes 10 or even 5) are simply not valid anymore. > > When trying to get into this field, its technologies and methods to tackle > the > data coming from them, then your best option is to actually work together > with > people who run a sequencing lab. They'll know the ins and outs and will be > able to provide you with literature which is not open to the public (vendor > documents often are not ... for whatever reason). And it's still interacting > with people you'll learn most. > > If you do not have such a link, then things get more difficult. Your first > stop > then are the technology vendor sites (454, Illumina, Pacific BioScience) who > have some general info regarding their sequencing technology. > > Your second stop: http://www.sequanswers.com/ > > It has developed to be a must read for everyone in the sequencing area. Tons > of question with an equal amount of answers ... many of them even quite good > as from knowledgable people. What you first should do is simply selectively > read postings from the last two or so years (reserve some time for that). A > couple of things should be clearer to you then. > > Afterwards, fell free to ask the very same question also on the SeqAnswers > board. Because I think that having a sticky post there tackling this kind of > information would be useful. > > B. > > -- > You have received this mail because you are subscribed to the mira_talk > mailing list. For information on how to subscribe or unsubscribe, please > visit http://www.chevreux.org/mira_mailinglists.html