[mira_talk] Re: mira- assembly jobs
- From: Visam Gültekin <teutara@xxxxxxxxxxx>
- To: Mira Talk <mira_talk@xxxxxxxxxxxxx>
- Date: Sat, 5 Mar 2011 16:46:35 +0000
Maybe it is not something common to post a message of this kind, but i really
appreciate your attention and rapid response Mr.Chevreux.
In fact i am a member of seqanswers and reading a lot from it.
Thank you, i will be following this talk-list, maybe some day i might answer a
question,
Visam GULTEKIN
> From: bach@xxxxxxxxxxxx
> To: mira_talk@xxxxxxxxxxxxx
> Subject: [mira_talk] Re: mira- assembly jobs
> Date: Sat, 5 Mar 2011 17:11:02 +0100
>
> On Wednesday 02 March 2011 12:42:58 Visam Gültekin wrote:
> > [...]
> > I am wondering what are the basic steps of this area, what kind of
> > instructions to follow, etc..? There are so many questions and most of
> > them should be answered by my own effort of course, but where to dig to
> > find the treasure?
>
> Actually an intersting question, I had hoped a couple of other people to come
> forward to say how they learned stuff about this topic. As no one else does,
> I'll start. But please be advised that part of the following is just a
> personal opinion.
>
> > I am trying to read articles about the topic i am asking, but there are so
> > many gaps. One tells about north, another from south. There might be some
> > cookbooks, some recipes that can be followed i think.
>
> Well, you basically hit the nail on the head with that observation: like
> cookbooks, recipes for sequence assembly come in tons of different flavours
> and
> there are a multitude of them. Unfortunately, unlike cookbooks, many of the
> underlying technologies change so rapidly that recipes which were valid 15
> years ago (or sometimes 10 or even 5) are simply not valid anymore.
>
> When trying to get into this field, its technologies and methods to tackle
> the
> data coming from them, then your best option is to actually work together
> with
> people who run a sequencing lab. They'll know the ins and outs and will be
> able to provide you with literature which is not open to the public (vendor
> documents often are not ... for whatever reason). And it's still interacting
> with people you'll learn most.
>
> If you do not have such a link, then things get more difficult. Your first
> stop
> then are the technology vendor sites (454, Illumina, Pacific BioScience) who
> have some general info regarding their sequencing technology.
>
> Your second stop: http://www.sequanswers.com/
>
> It has developed to be a must read for everyone in the sequencing area. Tons
> of question with an equal amount of answers ... many of them even quite good
> as from knowledgable people. What you first should do is simply selectively
> read postings from the last two or so years (reserve some time for that). A
> couple of things should be clearer to you then.
>
> Afterwards, fell free to ask the very same question also on the SeqAnswers
> board. Because I think that having a sticky post there tackling this kind of
> information would be useful.
>
> B.
>
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