Dear Bastian, Thank you for the miraSearchESTSNPs tip. For trying it I downloaded the 2.9.43 version. However, another problem appeared: Using identical input files and settings in the new version than in the 2.9.39 version, I get "floating point exception" when I try to do standard assemblies. Any idea what I do wrong? (to me this sounds rather like a bug...) Thanks again, R Roland Schafleitner PhD, Biotechnology Research Scientist Germplasm Improvement and Crop Enhancement Division International Potato Center - CIP Avenida La Molina 1895, Apartado 1558 La Molina, Lima 12, Peru Tel.: +51 (0) 1 349 6017, annex 3065