[mira_talk] * in padded file?
- From: "Shabhonam Caim (TGAC)" <Shabhonam.Caim@xxxxxxxxxxx>
- To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
- Date: Fri, 13 May 2011 09:43:21 +0100
Hi All,
Few basic beginner's questions:
1. Can anyone please tell me, what are the * character in padded file and
how is it going to affect the alignment. Do we consider the padded file or the
unpadded file?
2. The best way to assemble a transcriptome data?
I have used the following command to assemble the 454 reads:
mira-3.0.0 mira --project=test --job=denovo,genome,draft,454 454_SETTINGS
-LR:mxti=no -FN:fai=all_454_female_male.fna -FN:fqui=male_female.qual
but later I realized that it was a transcriptome data, can anyone help me out
in changing the parameters so that I can assemble the transcrpitome data.
Many thanks
Shab
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