Dear Bastien and All,
I really appreciate Mira. Thank you very much!
Today, I have a question about singlets.
After an assembly, if I check singlets in out.unpadded.fasta file, many of
those singlet reads are exactly same sequence.
So I am wondering why this is happening.
I have pasted my manifest information below.
I also uploaded input fastq files.
I would very much appreciate your help.
Best regards,
Lyu
Manifest file below
project = 10K
job = est,denovo,accurate
parameters = COMMON_SETTINGS
readgroup = SomePairedEndIlluminaReadsIGotFromTheLab
data = /mira_4.0.2_linux-gnu_x86_64_static/bin/10KR1.fastq
/mira_4.0.2_linux-gnu_x86_64_static/bin/10KR2.fastq
technology = solexa
autopairing
segment_naming = solexa
parameters = -SK:pr=90
parameters = -SK:mmhr=10
parameters = -SK:bph=32
parameters = -HS:mnr=no
parameters = -HS:ldn=no
parameters = -NW:cmrnl=no
parameters = -CO:mr=yes
parameters = -CL:ascdc=yes
parameters = SOLEXA_SETTINGS
parameters = -AL:mrs=90
parameters = -AS:mrpc=1
parameters = -AL:mo=15
parameters = -OUT:sssip=yes
Attachment:
10KR1.fastq.tar.gz
Description: application/gzip
Attachment:
10KR2.fastq.tar.gz
Description: application/gzip