----- original message -------- > I now use Mira since 3 months with 454 data. 1 month ago, I received > Solexa data (around 29M reads). I want to use these solexa reads to > correct/extend my 454 contigs. > I have an Out of Memory error: > [...] > But the strange thing is that if I check, Mira only used 4GB of Memory > on 24GB available Nope. Your system has 36 GiB. After loading the 29m reads, MIRA already used 32 GiB, 1 GiB was used by the OS and 3 GiB were free. > What am I doing wrong? Your machine has a bad set-up: only 4 GiB swap for 36 GiB RAM is not ideal. I'd go for a 1:1 setup there. Furthermore, you are operating at the limit of your machine there: miramem will tell you that you need around 36 to 40 GiB RAM for that project as minimum. Well, looks like you need a bit more than you currently have as RAM + swap. Tip: try mapping 20m reads to see whether you like what MIRA is producing with your data. B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html