[mira_talk] How much "log" space needed for Illumina de novo assembly?

  • From: Phillip San Miguel <pmiguel@xxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Thu, 12 May 2011 10:00:52 -0400

We have large amounts of NFS mounted drive space (>70 TB) but local space tends to be limited. Last night I attempted a de novo assembly of a quarter million solexa 101 base read pairs (5765742 total reads). This is a bacterial genome. I directed the "log" files to the local scratch disk, but the 167GB of available drive space were not enough.


How much "log" space will I need? Should I ask for the development version of mira be installed?

The possibly relevant parameters (other than -DIR and --project=) are:
--job=denovo,genome,accurate,solexa SOLEXA_SETTINGS -GE:tismin=200:tismax=700

This is MIRA V3.2.0 (production version).
Compiled by: bach
Sun Aug  8 18:43:42 CEST 2010
On: Linux arcadia64 2.6.27-11-generic #1 SMP Wed Apr 1 20:53:41 UTC 2009 x86_64 GNU/Linux
Compiled in boundtracking mode.
Compiled in bugtracking mode.
Compilation settings (sorry, for debug):
        Size of size_t  : 8
        Size of uint32  : 4
        Size of uint32_t: 4
        Size of uint64  : 8
        Size of uint64_t: 8
Current system: Linux gln 2.6.32-5-amd64 #1 SMP Mon Mar 7 21:35:22 UTC 2011 x86_64 GNU/Linux

The system I am currently running on is a debian linux dual quad core AMD Opteron(tm) Processor 2380 system with 32 GB of physical RAM.

Regards,
Phillip



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