Hi, Thank you very much John and Bastien for giving me reply. a) what version of MIRA Mira version 3.2.1.13 precompiled package. b) which operating system (output of "uname -a") Linux sandbox2. I am not the root user and i do not have permission. For installation i just downloaded the precompiled binary for linux (mira_3.2.1.13_dev_linux-gnu_i686_32_static.tar.bz2) , unpacked into my home directory: /home/comp/twlab/stamma/mira3.2.1.13/ Then i did 1. cd ~ 2. echo 'PATH=$PATH:/home/comp/twlab/stamma/mira3.2.1.13/bin' >>.profile 3. source .profile regards, Sravya Tamma,Washington University Medical School,St Louis Mo, 63108, From: bach@xxxxxxxxxxxx Subject: [mira_talk] Re: Error: while assembling Illumina paired end reads Date: Tue, 19 Apr 2011 01:12:02 +0200 To: mira_talk@xxxxxxxxxxxxx On Apr 18, 2011, at 23:32 , Sravya Tamma wrote:it gives me the following error in the log_assembly.txt file. "Could not stat the newly created directory 'Hpy_assembly'.: Value too large for defined data type That's a new one for me ... never seen this. Could you please tella) what version of MIRAb) which operating system (output of "uname -a")c) the directory you are starting the project in, is it something special (SAMBA share or similar)? B.