[mira_talk] Error: while assembling Illumina paired end reads
- From: Sravya Tamma <sravya12@xxxxxxxxxxx>
- To: <mira_talk@xxxxxxxxxxxxx>
- Date: Mon, 18 Apr 2011 16:32:15 -0500
Hi,
I am trying to assemble Illumina paired end reads of one of the Helicobacter
pylori genome. I have prepared the fastq file of paired end reads
(Hpy_in_solexa.fq) and placed it in bin/my_project folder.
But when i run the following command:
/mira3.2.1.13/bin/my_project$ mira --project=Hpy
--job=denovo,genome,accurate,solexa SOLEXA_SETTINGS -GE:tismin=100;tismax=300
>&log_assembly.txt
it gives me the following error in the log_assembly.txt file.
"Could not stat the newly created directory 'Hpy_assembly'.: Value too large
for defined data type
Creating directory Hpy_assembly ...
Fatal error (may be due to problems of the input data or parameters):
"Could not make sure that a needed directory exists, aborting MIRA."
->Thrown: void Assembly::ensureStandardDirectories(bool purge)
->Caught: main
Aborting process, probably due to error in the input data or parametrisation."
Could you please let me know where am i doing wrong?
Thanks,
Sravya Tamma,Washington University Medical School,St Louis Mo, 63108,
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